Cdk1, but not Cdk2, is the sole Cdk that is essential and sufficient to drive resumption of meiosis in mouse oocytes
نویسندگان
چکیده
منابع مشابه
Is cAMP decrease essential for resumption of meiosis in mouse oocytes?
Resumption of meiosis was inhibited in mouse oocyte cumulus complexes (OCC) co-cultured with pig membrane granulosa (PMG). After 3 and 6 h of co-culture these oocytes possessed an intact nuclear envelope and their nucleolar surface was associated with granules approximately 80 nm in diameter. Preincubation of OCCs for 30, 45, 60 or 90 min followed by co-culture with PMG for 2 h of either OCCs o...
متن کاملCdk2 activity is essential for the first to second meiosis transition in porcine oocytes.
The meiotic progression of Xenopus oocytes has been suggested to depend on the activity of cyclin-dependent kinase 2 (Cdk2). We examined whether Cdk2 is involved in the regulation of mammalian oocyte meiosis by injecting porcine oocytes with anti-Cdk2 antibody. At first, the cross-reactivity of the anti-Cdk2 antibody with Cdc2 kinase was evaluated by immunoprecipitation and immunoblotting exper...
متن کاملCdk1 drives meiosis and mitosis through two different mechanisms
Cell cycle progression in mammals involves multiple cyclin-dependent kinases (Cdks). Mice lacking Cdk2, Cdk3, Cdk4 or Cdk6 are viable, however, because Cdk1 can compensate for their loss by forming active complexes with A-, B-, Eand D-type cyclins in a stepwise manner. Thus, these Cdks are not essential for the mammalian cell cycle. In contrast, homozygous deletion of Cdk1 causes early embryoni...
متن کاملEffect of Fibroblastic Growth Factor on Resumption of Meiosis, In Vitro Maturation And Embryo Development of Immature Mouse Oocytes
Purpose: The purpose of this study was to evaluate the effect of fibroblastic growth factor on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without basic fibroblastic growth factor-4 (bFGF-4). Materials and Methods: cumulus – oocyte complex (COCs) and germinal vesicle (GV) were obtained from female NMRI mice 46-48 hours after adm...
متن کاملPnma5 is essential to the progression of meiosis in mouse oocytes through a chain of phosphorylation
PNMA (paraneoplastic antigen MA) family includes Pnma1-6. Although other members have been found to be involved in paraneoplastic neurological disorders, death receptor-dependent apoptosis, and tumorigenesis, Pnma5 was thought to be a female fertility factor, as indicated by one genome-wide study. But until now there have not been any further functional studies about Pnma5 in female meiosis. Ou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Human Molecular Genetics
سال: 2012
ISSN: 0964-6906,1460-2083
DOI: 10.1093/hmg/dds061